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Cyclic voltammetry displayed that the current of electrode increased 1.103 1.889 × 10−8 A after NaBH4 treatment in the different concentrations of target DNA.
Figure 6a, b shows AD recorded with the successive addition of aliquots of different concentrations of target cortisol and lactate antigen, respectively, over a wide analyte concentration range in a PBS buffer of pH 7.4, and Fig. 6c, d shows the respective calibration curves.
After 10 min, different concentrations of target DNA and 4 U of endonuclease Exo III were added to the sample solution.
Signals were taken after 30 min of incubation with 50 μL aliquots of different concentrations of Target T and other sequences, as described in the text.
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These trends were consistent with those from different concentrations of targets (data not shown).
The probe DNA-modified electrode was immersed into hybridization buffer solution containing different concentration of target DNA for 30 min at 37°C.
Flowing in different concentrations of fluorescent target proteins to the slide permits the very rapid identification and characterization of the binding properties of RNA aptamers obtained from a SELEX experiment.
The limit of detection (LOD) of the assay was determined to be 10 mg kg−1in a model cookie experimentally spiked with different concentrations of the target species.
In the quantitative analysis using different concentrations of the target virus, the absorbance logarithmically corresponded to the virus concentration in the range of 10 pg/ml to 10 μg/ml (Fig. 6C) and limit of detection was calculated as 50.5 pg/ml (Based on standard deviation method)45.
Different concentrations of the target are incubated in a constant concentration of the aptamer.
All the samples of the HPV genotyping microarrays with different concentrations of the target DNA of 0.05, 0.1, 0.25 and 0.5 pmol/μl are scanned by the photodiode based detection system.
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