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The different concentrations of standards (a mixture of cholesterol, cholesterol palmitate and triglycerides) were applied to each plate.
Interday and intraday precisions were done by preparing and applying three different concentrations of standards (in triplicate) on the same day and on three different days, respectively.
Calibration curves were generated using seven different concentrations of standards: 1, 5, 10, 25, 50, 75, and 100 mM for acetic acid (ACP, St Leonard, QC, Canada) and 0.6, 3, 6, 15, 30, 45, and 60 mM for lactic and butyric acids (Supelco, Bellefonte, PA, USA).
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Calibration curves were obtained for different concentrations of standard solutions prepared from 1000 mg/l stock solution (Merck, Germany).
For quality assurance, the analytical accuracy of the data generated in the laboratory was validated by repeated calibration using different concentrations of standard potassium hydrogen phthalate solution.
For CRP, the calibration curves of CRP were estimated with ten different concentrations of standard CRP, from 1 ng/mL to 250 μg/mL, diluted in CRP-free serum, when standard PCT was fixed at three different concentrations separately.
For PCT, the calibration curves of PCT were estimated with 11 different concentrations of standard PCT, from 0.2 to 300 ng/mL, diluted in PCT-free serum, when standard CRP was fixed at five different concentrations separately.
Intra-day and inter-day precisions were done by preparing and applying three different concentrations of standard in triplicate six times a day and similarly on six different days, respectively.
Different concentrations of standard, spanning from 0,114 ng/µl (0,5 µM) to 1,14 ngµl (5 µM) of 5-Aza-2-dC, were prepared and the peak area of each 10 µl sample was calculated by HPLC.
Standard curves were plotted using measurements taken for different concentrations of standard DNAs.
Recovery % values were obtained by the peak areas of the samples at three different concentrations of standard analytes.
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