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When β-gal was pre-incubated with different concentrations of free 1G40 phage prior to exposure to the biosensor, concentration-dependent inhibition was observed, indicating on biosensor high specificity toward β-gal.
b Cell viabilities of B16F10 cells after incubation with different concentrations of free PTX, PLP-131I, and FA-PLP-131I for 24 h.
Open image in new window Fig. 3 a The results of MTT assay after treatment of HT29 cell for 24 and 48 and 72 and 96 h with different concentrations of free SN38.
Cells were all treated with 2% DSS plus different concentrations of free raloxifene (2, 5, 10, 20 µM) or SMA- Ral (2, 5, 10, 20 µM), with one line of non-treated cells as a control.
After the incubation of Raji and Daudi cells with different concentrations of free ADR, rituximab Fab fragments, PC-ADR-BSA, and PC-ADR-Fab for 48 h, a CCK-8 assay was employed to determine the cell viability.
Briefly, cells were seeded in a 96-well plate at an initial density of 3,000 cells/well in 100 μL of RPMI-1640 supplemented with 10% (v/v) heat-inactivated FBS, 1% (v/v) antibiotics, and different concentrations of free ADR, PC-ADR-BSA, or PC-ADR-Fab or the corresponding concentration of rituximab Fab.
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In vitro viability of HeLa cells treated with a different concentration of free MMC and MMC loaded mPEG-FA-NPs after 24 h.
Fig. 1 cDNA samples (10 µl) of different inflammatory cytokines expressed by U937 cells, treated with different concentration of free or SMA Ral and visualized on 1.5% agarose gel.
To assess the impact of HMGB1 on the reactivity of immunocompetent cells in the presence or absence of the TNFα gene, spleen cells from TNFα+/+ mice and from knockout mice were stimulated with different concentrations of endotoxin-free pHMGB1 and the proliferative response was scored after 72 hours.
Among the remaining lipid fractions, no significantly different concentrations of glucosylceramides and free fatty acids were found among the body sites.
Figure 1A shows the effect of different concentrations of recombinant lipid-free apoE4 on the aggregation of TMR-labeled Aβ1 40.
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