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A set of 616 transcripts showing significant (false discovery rate <0.1) differences between the BMP4-treated Dicer-null and wild-type NS cells was hierarchically clustered to aid visualisation of the different classes of expression differences detected (Fig. 5J, K).
For validation qRT-PCR was performed for 20 randomly selected genes from different classes of expression levels and fold changes resulting in a significant correlation of r = 0.98 (Additional files 17 and 18).
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Breast cancer is a heterogeneous category of tumors, characterized by different classes of gene expression profiles [ 7].
Since several different classes of gene expression were changed upon OLIGO treatment, the mechanism behind it is presumably of general nature.
The analysis of these gene clusters indicates that the bzip1-1 mutriggersriggers at least four different classes of gene expression deregulation as follows: In the first class misregulated genes, in four cases (clusters #1, 6, 9, 10) the bzip1-1 mutattenuatesnuatesignalal regulation.
The comparative characterisation of histone modification profiles, DMR status and expression profiles for all confirmed imprinted genes provides a valuable resource for those interested in the role of these epigenetic marks in different classes of gene expression and repression and in the regulation of genomic imprinting in particular.
Sigma factors are thus key regulatory elements that control different classes of promoters and activate expression of the respective groups of genes (regulons or sigmulons).
One of the major factors which contribute to the grain yield, is the level of expression of different classes of yield-related genes which, in turn, is determined prima-rily by transcriptional regulation.
For the analysis of the effect of gene expression level on translational accuracy, we analyzed genes belonging to different classes of either breadth (EB) or level of expression (EL).
Genes that encode proteins which control basal transcription, including the five-subunit RNA polymerase core enzyme (α2ββ'ω) and σ-factors for binding specifically to different classes of promoters and hence selective expression of different groups of genes, are present in the L. hongkongensis genome.
Underscoring the lack of overlap between different classes of buffering QTL, for mouse gene expression traits we found differences in their mechanistic properties as well: ER QTL are mostly sex-specific and trans-acting, whereas GR QTL are not sex-specific and often cis-acting (the reason for this unexpected difference will be an interesting subject for future work).
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