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Different batches of data were used for adaptation and evaluation independently.
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But the actual error rate usually varies for different batch of data.
To account for interoccasion variation in experimental parameters between different batches of animals, data were expressed as percentage of control.
Averaged 2-D polypeptide maps were obtained using gels of three independent preparations, each from a different batch of plants (gels obtained with different root tip extract preparations isolated from different batches of plants were very similar; data not shown).
We speculate that RefOligo will improve comparison of data obtained from different batches of spotted arrays, by correcting for small variations in spot morphology and in the amount of spotted DNA across batches.
These controls were required to reliably compare data across different batches of samples.
A potential explanation is that this large set of experiments was conducted on different days and using different batches of plasma; these circumstances contribute to data variability, which makes it difficult to define a single set of parameter values to simultaneously fit all the data.
It has been clarified in the Methods (subsection "Lentivirus-mediated Caprin-2 knockdown in the SON and PVN") that Figures 3 and 4 represent data from 2 different batches of rats.
No significant differences were seen in different batches of resin.
The data are the average of three different batches of assembly reactions performed on different days.
For the electrophysiological experiments, data were collected from at least four different batches of neurons prepared on different dates, thereby minimizing the bias resulting from culture conditions.
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