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The images were obtained under different acquisition conditions of illumination and magnification.
On the other hand, extrinsic properties (i.e., properties that vary with different acquisition conditions) should not be captured, in order to maintain invariance to specific properties.
The method presents good performance even with noisy images and is robust to different acquisition conditions (illumination, camera settings, shadow, etc).
This method does not require to perform phantom acquisition for every different acquisition conditions and may serve to double check the calibration with an independent factor.
The effectiveness of the proposed indexes are proved by several tests, where a target is moved with an harmonic law in controlled condition (varying its frequency and amplitude) and fixing different acquisition conditions in terms of lighting settings, diaphragm aperture, exposure time, etc.
For example, in the low-risk group (H/M >2.0), none of the parameters had a meaningful impact on the numerical result, indicating that preserved myocardial uptake of I-MIBG can be reliably determined under widely different acquisition conditions.
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Since the complexity values are not dependent on the magnitude of the measured signal, the proposed method is less sensitive to the data sets measured under different data acquisition conditions.
However, its implementation can be quite different depending on the imaging modality, acquisition conditions, and desired transformation model [ 41].
In some cases extensive analysis at the microscope of a sample under different conditions is required to derive the optimal acquisition conditions.
Laser intensity in all these experiments was kept at the minimum and acquisition conditions maintained mostly unchanged over the different days of observation.
The intensities are indicated in arbitrary units (AU) since the acquisition conditions were not identical for the different samples.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com