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Therefore subtle differences in these mutants may not be discernable by the 'RIP1 laddering' assay in Figure 4C.
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In addition to the RNA blot data showing differences in these mutant lines, we have definitively shown that the flavonoid 3' hydroxylase is encoded by the T locus by sequence data of other alleles of the locus and by genetic cosegregation data [ 13].
The absence of obvious correlation between levels in lateral and central domains highlights qualitative differences in the mutants.
At first glance, the touch-sensing region looked normal, but on closer inspection the team noticed some subtle but persistent differences in the mutant mouse brains.
The nature of the difference in the mutants and how that confers 4-BPB-resistance, therefore, remain unknown.
Transcript levels were not perfectly correlated with IAA28 protein levels, suggesting differences in protein stability in these mutants.
The differences were the largest in the c4h and ccr1 mutants, although a large proportion of these differences in the ccr1 mutants could potentially be due to the altered development rather than to a compensation mechanism.
Cell proliferation and cell death analyses, carried out before the appearance of the ventricular anomalies, showed there were no differences in these parameters between control and mutants at stages immediately before abnormalities become apparent.
Significant deuterium exchange differences in SHP2 mutants relative to WT were mapped onto the SHP2 sequence and crystal structure in a heat map format.
Differences in mouse mutants might reflect observed phenotypes among patients with distinct RPE65 mutations (Cideciyan, 2010) (R. Ratnapriya, E.B., S. G. Jacobson and A.S., unpublished data).
One explanation for this difference in the mutant phenotype could be that Chlamydomonas is capable of synthesizing Chl in the dark unlike the angiosperms.
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