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The simulation results point to distinct differences in the viability of the cells at different scale of operation.
Differences in the viability of HMDMs after phagocytosis of particles sized 0.16 9.6 μm were statistically significant.
Nonetheless, further characterization of carbohydrates and proteins is required to account for the differences in the viability enhancing effect of the three IYDs higher molecular weight fractions.
Differences in the viability of A549 cells were considered significant at p < 0.05.
There were no significant differences in the viability of Caco-2 cells treated with PBS, rCTB-YVAD, or rCTB in the presence of LPS.
However, although the MTT assay has several limitations [ 18], we found it suitable for our study as it clearly shows systematic differences in the viability between sublines.
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The enhanced rhIL-4-induced IgE synthesis supported by C-IMDM was due to the combined effects of the added enrichment factors and not to differences in the viabilities of MNC cultured in C-IMDM and RPMI 1640.
Specifically, cell viability/cell death assays performed on NIH 3T3 cells showed no significant difference in the viability of cells in disk-containing samples versus disk-free controls after four days of incubation (Supplementary Fig. 7).
Also, no significant difference in the viability of cells can be observed between uncoated and BG-coated samples at 24 h of fibroblast culturing.
Compared to the PBS control, there was no statistically significant difference in the viability of cells that were treated with the particles at a concentration range of 0 to 100 μg/mL (p > 0.05), suggesting that the acetylated APTS-coated Fe3O4 NPs are noncytotoxic at the given concentration range.
To ensure the siRNA treatments were not having adverse effects on the cell, we performed cell viability assays and found no difference in the viability of cells treated with control pool siRNA when compared to spectrin, adducin or p4.1 siRNA treated cells (Figure S11).
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CEO of Professional Science Editing for Scientists @ prosciediting.com