The RT-qPCR results for chromosome alignment maintaining phosphoprotein 1 (CHAMP1) were used to calculate differences in the template RNA levels and thereby standardize the results for the genes of interest.
The qRT-PCR results for Chromosome 13 open reading frame 8 (C13orf8) [EMBL: AJ817183] were used to calculate differences in the template RNA levels and thereby standardize the results for the genes of interest.
The implementation of MULTICOM-RANK and MULTICOM-CMFR are the same as described above except a few minor differences in the template identification and ranking, and model generation steps (Table 1).
These DNA conformations correlated with two different positions of the loop between β strands 3 and 4. Importantly, although significant differences in the template position were observed, in both A and B molecules the primer strands were in the same position.
For instance, our group was able to perform the cross-modelling of two different murine MHC-I alleles, H2-Db and H2-Kb, demonstrating that our modelling procedure with Modeller software was able to reproduce all aspects of the MHC-I cleft, despite the known differences in the template (11).
Depending on the differences in template structure, template method can be divided into two groups called hard and soft template methods.
However, the result indicates that, with or without Chain Length Indexing, there are no significant differences in the length between templates (Table 5).
Because of considerable heterogeneity in the type of template used within this group and important differences in the items on different templates, we had an opportunity to describe variability in the selection of templates and also to test a hypothesis that the template type used to document care is associated with the documentation of processes of care.
Also, differences in the amount of template DNA and number of PCR cycles could account for the differences in sensitivity between these studies.
To correct differences in the amounts of template DNA, mRNA levels are shown as a ratio relative to the β-actin mRNA level.
