Sentence examples for differences in the template from inspiring English sources

The RT-qPCR results for chromosome alignment maintaining phosphoprotein 1 (CHAMP1) were used to calculate differences in the template RNA levels and thereby standardize the results for the genes of interest.

The qRT-PCR results for Chromosome 13 open reading frame 8 (C13orf8) [EMBL: AJ817183] were used to calculate differences in the template RNA levels and thereby standardize the results for the genes of interest.

The implementation of MULTICOM-RANK and MULTICOM-CMFR are the same as described above except a few minor differences in the template identification and ranking, and model generation steps (Table 1).

These DNA conformations correlated with two different positions of the loop between β strands 3 and 4. Importantly, although significant differences in the template position were observed, in both A and B molecules the primer strands were in the same position.

For instance, our group was able to perform the cross-modelling of two different murine MHC-I alleles, H2-Db and H2-Kb, demonstrating that our modelling procedure with Modeller software was able to reproduce all aspects of the MHC-I cleft, despite the known differences in the template (11).

If there is wide variation in template selection and also important differences in the templates, then future refinements of the system would need to consider selection of the template as an important factor in optimizing its effects.

Depending on the differences in template structure, template method can be divided into two groups called hard and soft template methods.

Also, differences in the amount of template DNA and number of PCR cycles could account for the differences in sensitivity between these studies.

To correct differences in the amounts of template DNA, mRNA levels are shown as a ratio relative to the β-actin mRNA level.

To normalise differences in the amount of template used, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript was used as an endogenous control (Pre-Developed TaqMan Assay Reagents Human GAPDH; Applied Biosystems).

C t values for the amplified target products and the internal control (β-actin) were determined for each sample, and the difference in C t for the target and internal control was calculated to normalise the differences in template and the efficiency of RT-PCR.