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This discrepancy was mainly due to differences in the stringency of the data-processing algorithm.
Differences in protocols, platforms, and number of replicate measurements can lead to significant differences in the stringency of the criteria used for detecting differential expression across experiments.
This was probably due to differences in the stringency of PQS screening as well as in length of target promoter region.
This discrepancy is likely due to differences in the stringency of read alignment and quality control cutoffs, as all of the breseq indel and SNV predictions appeared to be high quality upon manual examination.
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In the regular panning method, small differences were in the stringency of selection between different rounds of panning, but in modified subtractive panning after two or three rounds of selection, the antigen-binding clones showed a clear enrichment.
Another problem with microRNA microarray platforms is a divergence in the stringency of the detection call criteria.
At the same time, the Confucian tradition is unusual in the stringency of its duties to parents.
Differences in stringency of data screening parameters, the taxonomic composition of databases used for the comparative analysis, and methods of phylogenetic analyses can significantly affect the outcome of the study.
This is fewer than expected in comparison to previous reports on differential regulation during spontaneous Caco-2 differentiation and probably due to the difference in stringency of the algorithms used for data analyses (MAS 4.0 vs RMA), rather than related to functional biological discrepancies.
The liquid culture fluorescence data also confirm the decrease in fluorescence as the stringency of the GFP insertion construct increases.
In localization microscopy, the stringency of single molecule detection always influences the structural resolution.
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