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However, there are differences in the steps flanking the modified base pair compared to the same bases in the WT.
With so many differences in the steps of CPR it is much more challenging to remember the steps and the sequence of skills in infants/children than adults.
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There are significant differences in the step cycle compared with the sham group on day 7 (*p < 0.05) (C).
A cumulative effect would result in potentially non-statistically significant differences in the early steps of the process, with statistical significance reached at later steps of the process.
36 Hence, to detect a difference in the step counts a day, a minimum of 17 participants in each group is required to detect 80% power and maintaining a two-sided significance level at 5%.
These sample preparation workflows are all preceded by tissue deparaffinization and rehydration and usually comprise a high temperature treatment, but exhibit several differences in the other steps.
The importance of such an effect goes against dogma in the field: it was recently stated that 'The differences in the preprocessing steps for microarray data are immaterial... [when] the original classifier was developed using RT-qPCR' [ 36].
Inconsistencies among studies, regarding activity and levels of BER components, may be a reflection of tissue-specific differences in the individual steps in BER, for example in peripheral blood compared to the central nervous system.
> Following detailed examination of differences in the protocol steps for PB2 analysis, the timing of biopsy was found to be different between Bonn and Bologna, i.e. biopsy was carried out 2 3 h earlier in Bologna when compared with Bonn.
On the other hand, the differences in the map step are, as expected, much more significant.
Moreover, differences in the methanogenesis step were studied.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com