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There are significant differences in the step cycle compared with the sham group on day 7 (*p < 0.05) (C).
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However, there are differences in the steps flanking the modified base pair compared to the same bases in the WT.
With so many differences in the steps of CPR it is much more challenging to remember the steps and the sequence of skills in infants/children than adults.
36 Hence, to detect a difference in the step counts a day, a minimum of 17 participants in each group is required to detect 80% power and maintaining a two-sided significance level at 5%.
On the other hand, the differences in the map step are, as expected, much more significant.
Moreover, differences in the methanogenesis step were studied.
The gender differences in the mean step rate may be caused by differences in height and leg length.
The small differences in the first step between the two experimental setups are mainly due to the differences in the composition of the raw material used.
A cumulative effect would result in potentially non-statistically significant differences in the early steps of the process, with statistical significance reached at later steps of the process.
These sample preparation workflows are all preceded by tissue deparaffinization and rehydration and usually comprise a high temperature treatment, but exhibit several differences in the other steps.
The importance of such an effect goes against dogma in the field: it was recently stated that 'The differences in the preprocessing steps for microarray data are immaterial... [when] the original classifier was developed using RT-qPCR' [ 36].
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