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These data indicated that the differences in surface expression detected by flow cytometry were not attributable to differences in the steady state levels of protein expression or to differences in transfection efficiency.
The detection of HSF1 using a polyclonal anti-HSF1 antibody demonstrates that there are no significant differences in the steady state levels of HSF1 either treated or untreated with lambda phosphatase (Figure 1C).
Interestingly, we also found no differences in the steady state mRNA levels of the O3-increased KC and MIP-2 between Mmp9 +/+ and Mmp9−/− mice.
With the exception of aba1-1, no significant differences in the steady state levels of hsp90 were observed between EBR-treated and untreated mutant seedlings, including npr1-1 (data not shown).
For the strains which show differences in the steady state levels of Trk1-HA, we have not explored whether this occurs as a result of different rates of synthesis or degradation of the protein.
Quantitative real-time PCR (qPCR) was used to assess differences in the steady state mRNA levels of genes in response to the proteins of interest by comparison to a plasmid vector treated control.
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Nonetheless, we did not observe a difference in the steady state levels of this protein among the wild type and mutant Mtb strains.
The difference in the steady state behavior of the two motifs stems from the fact that the socialist motif has more negative feedback than the consumer motif: As negative feedback is increased, it becomes increasingly difficult to import an excessively abundant resource, and its intrinsic levels therefore become self limiting for the socialist [2].
Western blot analysis and quantification, after normalization against the GAPDH expression level, revealed no significant difference in the steady state level of the p.Glu119Glyfs*8 mutant protein compared with wild-type ATF6 protein (Fig. 3b).
There was no difference in the steady state levels of mutant CHC22 versus wild-type CHC22 when expressed in HEK293 cells, nor did this affect the stability of CHC17 (Fig. 3A and B).
However, ejections performed of Ru bpy 32+ in 5 mM and 150 mM NaCl displayed no difference in the steady state conformation (see the Supporting Information), indicating that this additional electroosmotic force was not a factor within the agarose.
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