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The ATR-FTIR spectra in Fig. 3d, by comparison, show many differences in the spectral features at different cellular locations.
A second possible reason or contributing factor for the disappearance of significant differences in the spectral peak wavelength among the different HuS concentration conditions after 24 h is that the protein corona evolved to a common composition over time for NPs incubated in the different serum conditions.
There are also subtle differences in the spectral shape of the response, discussed in the Supporting Information and in ref. 26, not relevant to the discussion here.
Polymerization of DABSA was also carried out to bring out the differences in the spectral characteristics between homopolymerization and copolymerization.
The differences in the spectral response functions and spatial resolution between relative spectral bands of the two sensors are attributed to these different performances.
First experiments involving the deposition of SiO2 layers in a thickness range from 15 nm to 45 nm on nanostructured TAC samples showed noticeable differences in the spectral reflectances obtained.
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Because the dichroic mirrors and emission filters are different for the two channels, and also due to the difference in the spectral ranges detected, each channel may have small differences in magnification, image rotation, shear, and other aberrations.
If that is the case, the difference in the spectral intensities between RA (R1, R2) and RB might reflect different amounts of degradation of the same original source material.
The insignificant difference in the spectral shapes and T1C values between PLLA-Q and PDLLA strongly suggests that the effects of difference in the chain regularity and interaction on the spectral shapes and T1C values are very low.
Less coherence in the observed phases of the ISO among various variables is due to the difference in the spectral components within the selected band.
Interestingly, after 24 h of incubation of the PEGylated AuNPs with cells, there was no longer a significant difference in the spectral peak wavelength among the 10, 25, and 50 % HuS conditions (Fig. 4).
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