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To correct for minor differences in the sequencing depth between specimens, the total number of tags was normalized to 106 for each specimen by dividing each tag weight by the total number of tags and multiplying this by 106.
Most of the re-sampled windows are expected to have no differences except for the differences in the sequencing depths.
There are significant differences in the sequencing methods, specimen preparation, run throughput and hands-on time between different sequencing platforms.
The large variation in the numbers of transcripts generated from the two assemblies stems from differences in the sequencing data and assembly procedures used.
This observation may support the hypothesis that mutations within the allele demonstrating a higher expression result in the differences in the sequencing patterns.
While these studies also analysed antennal transcriptomes, variations in the number of transcripts identified could arise from differences in the sequencing methods, sequencing depth, and/or sample preparation.
Similar(48)
The biggest obstacles to the direct comparison of data obtained from different microarray platforms are differences in the sequence and the number of probes that target each transcript.
Similarly, for Forkhead TFs FKH-1 and UNC-130, different methods produce variants with differences in the sequences flanking the core TGTTT Forkhead binding site.
Such an effect could be explained by a simple global inversion of ~5 Mb in the haplotype 4311 or by differences in the sequence of the different haplotypes.
When there were differences in the sequence, the researchers used a statistical model to predict the most likely original version.
Differences in the sequences could affect, for example, how likely a person is to have heart problems or suffer side effects from a cholesterol drug.
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