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However, there were no differences in the quantum of improvement of the FIM scores (P=.45) between the 2 groups.
The electronic unit was recently extended to record the three individual double coincidence ratios to take into account the differences in the quantum efficiencies of the three-photomultiplier tubes.
The use of DNA-sequencing instead of SNaPShot©-based primer-extension as the assay read-out obviates the gene-by-gene fine-tuning often required for SNaPshot assays, as well as the influences of differential efficiencies of incorporation of fluorescently labeled dideoxynuclotides into DNA and differences in the quantum efficiency of the fluorophores, which can distort AEI ratios.
The differences in the quantum yields between Trp42 and Trp68 in the N-terminal domain, and between Trp130 and Trp156 in the C-terminal domain, can be explained by the different rates of electron transfer from the indole ring to a local amide.
Additional measurement allowed us to estimate a background signal of 0.2 kHz in all channels, 4% crosstalk, and a γ-factor of 0.7, as a correction factor for differences in the quantum yields of the dyes and the wavelength-dependent detection efficiencies of the setup.
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While, the difference in the quantum yield owing to the difference in the measurement technique (O2 evolution vs. CO2 assimilation) can be appreciated, recent reports indicate that the quantum requirement for CO2 fixation in plants in the absence and presence of photorespiration is ~9 and ~ 10 photons, respectively [ 83, 84].
17 Since the positions of the phosphorescence maxima of the IrIII benzoporphyrin and the IrIII corrols are similar, such a huge difference in the quantum yield cannot be explained by the lower energy gap between the triplet excited state and the ground state for the corrols (λmax ≈ 790 nm).
Tsyboulski et al. [3] compare the emission of two different layers of a DGU column to determine the difference in the PL quantum yield of DWCNT compared to SWCNT.
The difference in the molar quantum yield between fluorescein conjugated to calibration particles and to antibody molecules was not taken into account.
These characteristic energy values, defined by the differences in the energies of the quantum states, are responsible for atomic spectral lines.
Furthermore, to determine the actual FRET efficiency, another instrumental correction was made through the correction factor γ, which accounts for the differences in quantum yield and detection efficiency between the donor and the acceptor.
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