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The observed differences in the quantification data discussed previously would lend weight this hypothesis.
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Although no significant difference in the quantification of the pimonidazole staining was detectable, a qualitative trend of decreased staining density was observed in the long-term study (see Figure 5).
This is possibly related to the difference between the studied populations or the difference in the quantification of detached glycans and glycopeptides.
When the difference in the quantification cycle (Cq) value of the two counterparts was >1, the sample processing was repeated in another qPCR set until the Cq difference was <1 cycle to ensure concordance and accuracy.
While no significant difference in the quantification of synovial F4/80 staining was observed in synovial tissue for WT and IL-10KO mice, an increased localization of F4/80 staining was observed around sites of focal bone erosions in sections from AIA-challenged IL-10KO mice.
Does pharmacological blockage of Ptch1 endocytosis/degradation affect Ptch1 ciliary exit? 4) The effect of Δ2PY-GFP on Smo ciliary localization presented in Figure 4 does not match the quantification well, especially at 4 hours after Shh treatment where there is almost no difference in the ciliary Smo levels between Δ2PY-GFP and Ptch1-GFP while there is a 2-told difference in the quantification.
Furthermore, there are differences between studies in the quantification used to define the 'binge' pattern; for women, the National Institute on Alcohol Abuse and Alcoholism (NIAAA National Advisory Councill and the Centers for Disease Control and Prevention define the consumption of ≥4 drinks on a single occasion as binge drinking [ 17].
Inherent technological differences in the detection and quantification processes between the techniques probably caused some inconsistency between the data sets.
Specifically, dCt values were normalized and approximations to −2 for the lower values and 5 for the highest were applied in order to highlight the differences of the quantification values in the mid-region.
This is due to the difference in quantification methods, the absolute quantification of integrated HIV-1 DNA being corrected by using an integration standard as calibrator as previously described (Liszewski et al., 2009; De Spiegelaere et al., 2014).
A brief discussion of the seeming discrepancy between the differences in quantification may help the reader.
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