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When comparing miRNAs across different species, we found minor differences in the nucleotide composition of the miRNAs of some species.
Although it is known that there are large differences in the nucleotide composition of mitochondrial sequences of different lineages as well as in the amino acid composition of the encoded proteins, this bias is often not considered in phylogenetic analyses.
Because there are large differences in the nucleotide composition of mitochondrial sequences of different lineages as well as in the amino acid composition of the encoded proteins [ 42- 48], we applied several approaches for reducing compositional bias in the phylogenetic analyses.
Our findings showed that though there were differences in the nucleotide sequences in the nsP1 region, there was 100% amino acid homology.
The ITS2 secondary structures obtained are the same; however, they differ in terms of MFE, − 242.60 kcal/mol (H71D strain) and − 245.30 kcal/mol (L. ramosa GQ342874), due to the three differences in the nucleotide sequence.
Of note, there were individual differences in the nucleotide sequences not resulting in exchanged amino acids.
Similar(28)
In addition, other noticeable differences in the nucleotide-binding N-terminal domain of the various Hsp70s may also affect the functional role of these proteins.
A difference in the nucleotide sequence of a gene will result in the difference in the nucleotide sequence of RNA molecules, which in turn can result in a difference in the amino acid sequence of a polypeptide.
Allelic imbalance (AI) is a difference in the nucleotide frequencies at a given genomic position between two RNA samples.
An example is allelic imbalance (AI), which we define here as a difference in the nucleotide frequencies at a given transcriptome position between two tissues/conditions (Figure 4a).
For each exon, there was no statistical difference in the nucleotide accessibility and engaged scores computed from secondary structures predicted based on different sequence lengths of window of analysis (data not shown).
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