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The absence of obvious correlation between levels in lateral and central domains highlights qualitative differences in the mutants.
Thus, evaluating this set of mutants provides a potentially sensitive analysis for subtle effects of loss of exocyst function, i.e. small differences in the mutants can be considered more credible when the magnitude of those differences consistently correlate with the severity of the root growth defect across all mutants evaluated.
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Indeed, our results emphasize this important fact, since not only did we find differences in performance between various wild type genetic backgrounds, we also saw dramatic phenotypic differences in the mutant strains carrying the same mutation but in a different genetic background as analyzed in detailed secondary screens.
At first glance, the touch-sensing region looked normal, but on closer inspection the team noticed some subtle but persistent differences in the mutant mouse brains.
The three populations showed significant differences in the mutant allele frequency in co-culture (ANOVA: F2,12 = 41.242, p < 0.001), indicating that genetic background also has an influence on competition outcome.
Scanning electron micrographs and light microscopy photographs (Figs. 2 and 3, respectively) show the morphological differences in the mutant strains when compared to the wild-type strain and to one another.
The nature of the difference in the mutants and how that confers 4-BPB-resistance, therefore, remain unknown.
The differences were the largest in the c4h and ccr1 mutants, although a large proportion of these differences in the ccr1 mutants could potentially be due to the altered development rather than to a compensation mechanism.
3) The last section of the Results deals with sex differences found in the mutants (males were worse than females).
If so, is there a fixed distance across environments and mice? 3) The last section of the Results deals with sex differences found in the mutants (males were worse than females).
There is essentially no difference in the mutant and wild type miRNAs with respect to 'si' or 'mi' function and none would be predicted from the position of the alteration.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com