Then, we trained a modified predictor (using the same genes but creating a different model due to technical platform differences) in the largest U133 dataset (JAPAN), and directly applied it to the remaining datasets (UK and MSKCC) and vice versa.
Without support in the Senate, the fate of the amendment lies with what's called a conference committee made up of Senators and Members of the House who will hash out the differences in the larger CJS bill, including the marijuana component.
This emphasizes the importance of hybridizing all samples together when microarrays are used to look for biological differences in the large-scale organization of gene expression levels.
We use data from the MicroArray Quality Control Project (MAQC) to demonstrate that our method is robust and that it reliably detects biological differences in the large-scale organization of gene expression levels.
Despite the presence of effects of hybridization, we also showed using the MAQC data set that biologically related differences in the large-scale organization of gene expression levels are consistently and reproducibly detected.
We found in multiple microarray experiments that differences in the large-scale organization of gene expression levels in the hippocampus, rather than specific differences in gene expression levels, were associated with individual variability in hippocampus-related behaviors.
Similarly we found even in the presence of technical effects of RNA processing method that biologically related differences in the large-scale organization of gene expression levels associated with postnatal handling were detected using either method of RNA processing.
Because the authors examined gene expression in both the hippocampus and the amygdala, we were also able to address the question of regional specificity of differences in the large-scale organization of gene expression levels that are associated with behavioral variability.
In order to quantify possible cumulative differences in the large-scale organisation of the heterochromatin of chromosome 1 in ICF B-cells and controls, we measured the intra-nuclear areas occupied by the juxtacentromeric heterochromatic regions, as defined by hybridisation on 2D-fixed interphase nuclei with the corresponding classical satellite DNA probe (Fig. 2).
