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The data reveal striking differences in the internalization behaviour and gene silencing efficiency in the tested cell lines, despite their common lung epithelial origins.
In addition, marked differences in the internalization efficiency of the 18F-labeled EuE-based inhibitors in comparison to the two reference compounds 18F-DCFPyl and 18F-PSMA-1007 were observed (Fig. 2a).
Differences in the internalization of F-Aβ40 and AF633-Trf, controlled for temperature and energy dependent transport, was also assessed using laser confocal microscopy and flow cytometry.
The differences in the internalization kinetics may suggest that ES and its hybrid variants use different endocytic routes.
Contrary to CysLT2, LTD4 did not induce internalization of hGPR17-L as only constitutive internalization was observed when either this or vehicle was present with no apparent differences in the internalization pattern between these conditions.
Although cross-linking of FPR molecules at the cell surface by the antibodies is likely to enhance receptor internalization, this approach could still allow the detection of clear differences in the internalization between stimulated and non-stimulated cells.
Similar(54)
Such a phenomenal difference in the internalization of Aβ40 between neurons and BBB endothelial cells may provide essential clues to understanding how various cells can differentially regulate Aβ proteins and help explain the vulnerability of cortical and hippocampal neurons to Aβ toxicity.
We found that there was no significant difference in the internalization of solid discs by J774A.1 macrophages compared to solid spheres after 2 h of incubation.
During the first 10 min of the internalization period, there were no significant differences in the internalized GluR2 between the Tmub1/HOPS-RNAi-transfected and scramble-transfected neurons (n = 12, P>0.05; t-test; Figure 5B).
We observed no statistical differences in the adhesion and internalization indexes when we used pre-treated parasites with dynasore 60 and 100 µM before interaction with non treated host cells (Figure S1).
Although there might be differences in the dynamics of internalization by artificially crosslinking BCRs with agonistic antibodies versus antigens that naturally crosslink the BCR [33] this seems of minor importance for our study comparing the transcriptional responses to various antibody treatments.
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