However, there was no significant difference in the fertilization and embryo developmental competence in relation to the hCG priming time.
However, most of the other studies did not report a significant difference in the fertilization rates between the obese or overweight women and the normal women [ 15, 20, 21].
In line with the available literature, the current study showed no difference in the fertilization rate per mature oocyte, positive pregnancy rate per cycle or clinical pregnancy rate per cycle between the groups.
To demonstrate the non-inferiority based on an absolute difference of 17% in the fertilization rate per sibling oocyte, a minimum of 222 oocytes were required.
Similarly, both leaf nitrogen uptake and leaf biomass presented vertical bell shape distribution, although only the former showed qualitative differences among the fertilization treatments in the intermediate canopy leaf levels.
Experiments designed to directly examine in vivo pollen tube growth rates indicate that these differences in the ability to achieve fertilization under competitive conditions are due to differences in pollen tube growth rates.
A longer hatching time, however, does not necessarily imply differences in the time span between fertilization and hatching.
Whereas species with internal fertilization can tolerate differences in the maturation of each partner, successful fertilization in such broadcast spawners critically relies on tight synchronization between male and female maturation and the release of germ products.
The differences in fertilization success ("brother" minus non-sib male) were calculated for each population and used as dependent variable, the sub-trial (main or control experiment) was included as explanatory variable and trial number was defined as random factor and never removed to control for the paired study design.
When compared with the untreated group, no differences in fertilization and subsequent development to the blastocyst stage were observed (p>0.05; data not shown).
