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We refer to the larger element as a chromosome and the smaller one as a megaplasmid largely due to the differences in the array of predicted functions encoded by their respective genes.
Together, these data indicate that the variation in Wolbachia prevalence among populations can be explained largely by differences in the array of Wolbachia variants within host populations.
Thousands of expression studies employ one or the other microarray platform, but comparison of results between platforms has been difficult because of inherent differences in the array technologies.
There are some differences in the dominant vibration direction between forest machines, snowmobiles and snowgroomers (Table 1), which may be an explanation for the small differences in the array of neuromusculoskeletal disorders between the driver categories in this study.
This may result from differences in the array type used (Affymetrix MG-U74Av2 vs. Rat Gene 1.0 ST), the number of annotated genes investigated (~9, 000 vs ~28, 000), the statistical analyses performed (Wilcoxon signed rank test vs. moderate paired t-test) or species (mouse vs. rat).
Each of these mutants was selected because it exhibits significant residual catalytic activity [indeed, V329A exhibits enhanced catalytic activity (see Table 3)] and/or interesting differences in the array of resulting sesquiterpene products compared to the wild-type enzyme (Table 4).
Similar(53)
Although there was evidence of substantial difference in relative intron expression across arrays, it was not clear to what extent this reflected biologically significant differences between the samples or merely differences in the arrays or sample preparations.
In order to validate the expression levels observed in the arrays, we selected 4 genes for quantitative real time PCR analysis (Q-RT-PCR): 2 regenerating islet-derived coding genes, Reg2 and Reg3a and the 2 insulin genes, Ins1 and Ins2 that have shown high gene expression differences in the arrays [see Additional File 3].
We found no evidence for hemispheric differences in gene expression levels for either of the miRNAs investigated (Table 1), which suggests that the minor right/left differences seen in the array data represent false positive findings.
The difference in the arrays -12K vs 21K arrays used in our study- the relative paucity of the targets identified (100 vs 136) and the difference of the cellular context –the MCF12A mammary cell line transfected with C/EBPδ vs normal keratinocytes- might account for this finding.
These differences could be due to technical differences in the cDNA array platforms, to the methods of analysis used in each study, or (most probably) to patient inclusion criteria.
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CEO of Professional Science Editing for Scientists @ prosciediting.com