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b The CCK-8 assay shows that there are significant differences in the absorbance of cardiac fibroblasts cultured on collagen and CNH-Col substrates at different stages.
As observed in Figure S23, the differences in the absorbance in the presence and absence of RBCs are not significant, indicating that NPs remained in the solution for both cases.
Note that there are quantitative differences in the absorbance values, and these reflect the fact that when substrates mixed in a single well, then the amount of each individual substrate is proportionally lower since the total volume of substrate material is the same in each well.
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Measurements with filtometers are based on the difference in the absorbance before and after casein precipitation.
This explains the difference in the absorbance readings of the controls of both groups.
The absorbance was measured at 450 and 720 nm using a spectrophotometer and the difference in the absorbance readings is expressed as mAU.
UV/VIS/NIR spectrometry combined with static positioning (2.5-mL NCBNF in a transparent cuvette) was performed to measure the difference in the absorbance of the initial (Abs i ) and static position of each NCBNF after 8 h (Abs t ).
Although there was no significant difference in the absorbance produced at 50°C and 60°C (i.e. 0.390 and 0.398 at 5 min; 0.395 and 0.4 at 20 min, respectively).
The difference in the absorbance before and after centrifugation gives the amount of peptide aggregated.
However, since we were interested in the difference in the absorbance from the day of euthanasia to the day of autopsy for each individual, the detected species difference in OD values should not represent a bias in this study.
As for the pressurization time, there was no significant difference in the absorbance level among the pressurization times of 5, 10, 30, and 60 min for the same pressurization group at both 100 and 200 MPa.
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