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Furthermore, it cannot be excluded that differences in solutions used for mock infection (Hanks balanced salt solution in the study by Little et al. [ 19] vs. SPG solution in the present study) may have affected the final outcome.
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Skrynnikov, N.R. et al. Orienting domains in proteins using dipolar couplings measured by liquid-state NMR: differences in solution and crystal forms of maltodextrin binding protein loaded with beta-cyclodextrin.
Furthermore, we see the significant differences in solution structures.
Smaller size of particle may be caused by differences in solution charge.
However, we must consider the possibility that differences in solution saturation conditions could not be identified because the reactive pore water was not sampled in the Al-CSH layer.
All mixtures of meat and gastric juice were then adjusted to pH 3 with HCl to minimise effects caused by slight differences in solution pH during this extraction step.
This suggests possible subtle structural differences in solution compared to the single crystal.
Previous work has explored these differences in solution [ 7] and in silico [ 8] using solution-phase algorithms, but solution-phase kinetics do not translate well to solid phase hybridization [ 9].
However, because the two enzymes share only partial sequence identity, it was important to be certain that differences in solution hydrogen exchange were not due to differences in structure between the two enzymes.
Although the asymmetry in the structure of yeast ADH1 could result from inherent structural differences in solution, there is no evidence of "negative" cooperativity in the binding of coenzymes or the kinetics of catalysis.
The reason is that we wanted to guarantee that differences in solution quality are caused by the fact whether or not and how the additional information was used in the optimization and not by conceptual differences between the underlying optimization methods.
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