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This analysis also served as a control to clarify that the generalization of multi-voxel patterns is not decreased due to methodological constraints such as differences in realignment of fMRI data from the different days.
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Previous workers have demonstrated that there is no significant difference in the postoperative realignment of the cervical spine between ACDF and HDF in the management of 3-level CSM [ 22].
We tested for group differences in head motion parameters from image realignment by comparing the framewise displacement and the root mean squared movement (Power et al., 2012; Satterthwaite et al., 2013).
Preprocessing included correction for slice-timing differences [18], realignment to compensate for bulk head motion, normalization to standard MNI space using an in-house developed EPI template optimized for 7 T EPI imaging.
A slice timing correction was applied to correct for differences in image acquisition time between slices, and realignment was performed to minimize movement artifacts in the time series (Unser et al. 1993a, b).
Over the years, the WAC has been front and center in realignment.
VBS showed its strengths especially in realignment of crush and biconcave fractures.
The remaining functional images were corrected for differences in slice acquisition timing, which was followed by realignment to the middle image in the initial scan to correct for inter-scan movement and to remove signals correlated with head motion.
This analysis included standard preprocessing procedures: realignment, slice timing to correct for differences in slice acquisition time, normalization (images were normalized to an EPI template), and smoothing (with an isotropic 8-mm FWHM Gaussian kernel).
We observed differences in the masking of repetitive sequences in Applied Biosystems vs. Illumina realignments, suggesting that repetitive regions are problematic for both platforms with short read sequencing.
We modelled the six realignment parameters as effects of no interest to account for any group differences in head movement.
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