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Analogous to bulk PPEs, this model system shows dramatic differences in quantum yield between the folded and unfolded states, with the unfolded urea having greater than 400-fold higher fluorescence quantum yield than its folded equivalent.
Furthermore, to determine the actual FRET efficiency, another instrumental correction was made through the correction factor γ, which accounts for the differences in quantum yield and detection efficiency between the donor and the acceptor.
For a more generalized comparison between sdLCS and tdLCS, we take into account the differences in quantum efficiency, scan speed and scan range of the two methods (λ0 and λBW are equal since we employ the same light source): with ΔLmax the maximal measurable path length difference by the spectrograph after removal of the DC and modulation terms.
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On the face of it, the only difference in quantum gravity is that spacetime itself becomes a kind of quantum field, and one would perhaps be inclined to say that the properties of spacetime become indefinite.
This difference in quantum efficiency is may be due to conjugation through aromatic system as well as strong hydrogen bonding between nitro of 7d and ethanol, and thiazole of 7e and ethanol.
Table 4 Sensitivity coefficients for the four channels of the 1-μm Channel Sensitivityitivity coefficient* 0.90 µm (day) 61.7 ± 4.7 0.90 µm (night) 0.0756 ± 0.0058 0.97 µm 0.608 ± 0.070 1.01 µm 1.35 ± 0.91 The difference among nightside sensitivity coefficients as large as a factor of ten seems to come mostly from difference in quantum efficiency.
On the contrary, free Nap 2 reporter is strongly fluorescent, with a quantum yield (Φreporter) of 0.19 in the aqueous medium used here, resulting in a 64-fold difference in quantum efficiency versus the HMBQ-Nap 1 probe.
Nor do any differences exist in quantum yield among species (F3,19 = 1.883, P = 0.173, data not shown).
However, there were no differences in the quantum of improvement of the FIM scores (P=.45) between the 2 groups.
The electronic unit was recently extended to record the three individual double coincidence ratios to take into account the differences in the quantum efficiencies of the three-photomultiplier tubes.
The RFd-images (RFd = (Fp-Fs)/Fs) as indicators of photosynthetic activity allow to evaluate differences in photosynthetic quantum conversion.
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