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To minimise systematic differences in quantification, three measures were taken.
Conclusion: Integreation of age and gender differences in quantification of DaTscan is feasible and applicable in clinical routine.
Several studies have compared the PET images from PET/CT and PET/MR in clinical data [5 17], and the vast majority have demonstrated equal diagnostic performance and detection rates, despite some differences in quantification.
A brief discussion of the seeming discrepancy between the differences in quantification may help the reader.
A possible reason for the inconsistent findings could be related to the methodological differences between investigations including differences in quantification of disability (e.g., self-report versus clinically determined) and cognitive task utilized.
We speculate that the differences in quantification methods contribute to gene-to-gene variation and unspecific noise, but not to systematic variation that would affect samples from separate tissues in different ways.
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Discordant results (33% difference in quantification or positive/negative results) were read by a third microscopist; agreement between any two micoscopists and the average parasite density were deemed to be the correct finding.
Additionally, for in-house assays at sites 3 and 5, the difference in quantification compared to the CAP/CTM CMV test varied throughout the measuring range.
This is due to the difference in quantification methods, the absolute quantification of integrated HIV-1 DNA being corrected by using an integration standard as calibrator as previously described (Liszewski et al., 2009; De Spiegelaere et al., 2014).
In practice, no significant difference in quantification of malignant lesions is found comparing CT-based attenuation correction with segmented AC (SAC) using transmission imaging [ 28] or between measured (MAC) and segmented (SAC) attenuation correction using transmission imaging [ 29].
A recent study by Westerterp et al. showed differences in SUV quantification between institutes with different PET scanners [ 26].
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