The results from repeated analyses showed a difference less than 5% in all the cases except one, which showed difference as large as 18%.
We highlight in Supplementary Fig. 3 regions that exhibit differences in all three cases: the whole data set, the first half, and the second half.
By applying Kruskal-Wallis test, we found that there existed differences in all the 16 test cases.
In all the cases, the differences between those which were positive for OA, according to our KHOA-SQ, and those who were not, were statistically significant with p < 0.001 in all the conducted comparisons.
Nevertheless, in all the cases, only minor differences were obtained.
In our hands, internalisation of TRAIL receptors ligated by agonistic DR4 and DR5 antibodies or by rhTRAIL revealed no significant differences; in all cases, the ligated receptor complex was rapidly internalised.
Comparing the profile of background substitutional patterns derived from various methods of phylogenetic reconstruction (maximum parsimony, maximum likelihood, and neighbor-joining) yielded no significant differences; in all cases the estimates of individual rates of single-nucleotide substitutions were virtually identical (P > 0.2, all comparisons, two-tailed t-test).
These values were already higher after the first session with the psychologist, taking into consideration the two populations separately (hip and knee), with significant differences in all cases, apart from the pre-operative stage for the patients undergoing knee operations.
Pairwise comparisons between clusters also showed significant differences in all cases, except for the following: cluster 1 versus cluster 2 in regard to GAD-7 (P = 0.96) and PHQ-9 (P = 0.07) and cluster 3 versus cluster 4 with respect to PHQ-9 (P = 0.53).
Pairwise comparisons between clusters also showed significant differences in all cases, except for the following: cluster 2 versus cluster 3 MOS-Sleep Problems Index II (P = 0.16) and cluster 1 versus cluster 2 with respect to both POMS Tension-Anxiety (P = 0.26) and Depression-Dejection (P = 0.23).
