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The differences between the protocols of the six DNA extraction techniques are described in Table 1.
Ordinal regression analysis was applied to further identify differences between the protocols and their effect on the quality of the images produced and therefore as predictors of image quality.
We considered that it may be dependent on the doses and / or the differences between the protocols of treatments used.
The results of these two studies are contradictory and difficult to compare due to marked differences between the protocols.
The most important differences between the protocols and machines currently used are blood volume (from 9 to 120 mL), PRP volume (from 3 to 32 mL), activators (CaCl2, thrombin, batroxobin, bovine thrombin, and thrombin added to CaCl2), number of spins during centrifugation (1 or 2), and PLT concentration (from 1x to 18x) [ 141, 142].
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Fig. 3 Differences between the protocol of Yamanoi et al. and our revised protocol.
The main differences between the protocol of Yamanoi et al. and our revised protocol are illustrated in Fig. 3.
The differences between the protocol used here and the usual procedure relate to culture time (4 instead of 3 days) and to the timing of the irradiation.
12 We have found that some differences between the protocol and the publication are a result of outcome reporting bias, but also that some changes are made during the course of the trial as a result of poor outcome choice at the protocol development stage.
One significant difference between the protocols in the two studies was that, in the first study, animals were maintained in silence following 2-DG injection, whereas in the Tucci et al. study, animals were exposed to sound.
MHS method seems to be noisier which can be an effect of the difference between the protocols or conditions of the labs where the arrays were hybridized.
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