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Serial sampling method was used until a desired sample size was reached and the sample size for this study was calculated by using a formula for a difference proportions (equal sized groups).
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Differences, proportions, and correlations are reported as point estimates, bounded by 95% confidence intervals (CI).
When proportions were being compared, a two-tailed z-test for the difference between two proportions was performed assuming unequal group variances as described by AP Statistics Tutorial (http://stattrek.com/AP-Statistics-4/Test-Difference-Proportion.aspx).aspx
Proportions were compared by calculating 95% CI for the difference in proportions [ 34].
Tests for difference of proportions were conducted to evaluate the significance of genotype subgroup proportion differences in MS criteria.
Differences in proportions to verify differences between countries for other descriptive variables were analyzed using Chi-tests.
Several methods exist to calculate sample size for the difference of proportions (risk difference).
This computation assumes that the difference in proportions is −0.12 (specifically, 0.20 versus 0.32).
However, the difference in proportions was not statistically significant.
However, the difference in proportions of Reverters for both antigens was not significant (p = 0.26).
Difference in proportions was assessed using gee-logistic regression.
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