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Secondly, this could be due to the difference in the strains of the toxoplasma and the use of in-house PCR primer.
There was also a significant difference in the strains resistance to inhibition: four of the intruders were not susceptible to inhibition by any of the resident strains and the remaining strains were inhibited by 1 5 strain(s) (on average two; Table 4, nested model: p<0.0001).
Another possibility why we could not replicate Obokata's results might be the difference in the strains of Oct4-GFP transgenic mice used.
The observed 5.7-h-later time at which N2 first reproduced accounts for the calculated difference in the strains' population doubling times, 12.1 h for N2 and 11.4 h for MY6.
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Numerical modelling showed that this difference in the strain field could be explained by the different orthotropic elastic and frictional properties of the two materials.
This large order of magnitude difference in the strain rates prevents a direct one-on-one comparison between experiments and simulations.
Deformation fields are measured using full-field digital image correlation (DIC) to reveal a large difference in the strain localisation behaviour of the material depending on the amount of lateral deformation.
This seems to be the reason behind the difference in the strain values between the experimental and FEA models.
This difference cannot be ascribed simply to a difference in technique because, apart from a difference in the strain of rabbits (the previous study used NZ white rabbits while we have used Dutch-belted), the methods are essentially the same.
Another possible explanation is the difference in the strain background.
Strain typing identified 3 quarters where a difference in the strain was observed between pre-treatment and post-treatment isolates.
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