It was previously reported that the difference in the scattering intensity I q) between the end-modified and the non-modified SBR can be observed at a q-range of 0.0015 0.06 nm−1.
These misfits are probably due to local difference in the scattering and intrinsic absorption factors and/or directionality due to radiation pattern, which are not reflected in the used envelope Green's function.
Consequently, for the ratios of total scatter, and side scatter to vary greatly, there must be a huge difference in the light scattering from mitochondria and lysosomes in the near forward or near backscattering directions.
The objective here is to investigate if any amplitude dependence in this lower range of loading can explain the observed differences in the scattering of these two modal parameters.
To better visualize the trend of allele frequency differences in the scatter plots, we drew LOESS regression lines in the plots (Minevich et al. 2012) by using Matlab's smooth function with the span parameter set at values corresponding to 60 kb (0.0108 for chromosome I, 0.0132 for chromosome II, and 0.0245 for chromosome III).
N is the number of independently scattering particles, Δρ the difference in the scattering length densities of the particles and the solvent, and V corresponds to the volume of each of the particles.
The maximum reduced scattering coefficient for tissue samples is 1.19 mm-1 at 635 nm, and the minimum reduced scattering coefficient is 0.521 mm-1 at 980 nm, and the maximum difference in the reduced scattering coefficients is 128% between 635 and 980 nm, and the minimum difference is 1.15% between 890 and 980 nm.
Caution must be taken, however, as these differences could have been due to general differences in the light scattering properties of Ucp1KO and wild-type mitochondria that were unrelated to mPTP.
Outside the LPZ, only the global mapping method revealed a significant difference in the CRRFs' scatter between the lesioned and the control eyes (control = 0.11 mm, lesioned = 0.16 mm, Fig. 11 C).
