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There is essentially no difference in the mutant and wild type miRNAs with respect to 'si' or 'mi' function and none would be predicted from the position of the alteration.
One explanation for this difference in the mutant phenotype could be that Chlamydomonas is capable of synthesizing Chl in the dark unlike the angiosperms.
This difference in the mutant screening yields highlights the instability of the hnwd genes as opposed to the repeat-free het-s, het-S genes, het-c or modA genes [ 48].
Similar(56)
The nature of the difference in the mutants and how that confers 4-BPB-resistance, therefore, remain unknown.
Indeed, our results emphasize this important fact, since not only did we find differences in performance between various wild type genetic backgrounds, we also saw dramatic phenotypic differences in the mutant strains carrying the same mutation but in a different genetic background as analyzed in detailed secondary screens.
At first glance, the touch-sensing region looked normal, but on closer inspection the team noticed some subtle but persistent differences in the mutant mouse brains.
The three populations showed significant differences in the mutant allele frequency in co-culture (ANOVA: F2,12 = 41.242, p < 0.001), indicating that genetic background also has an influence on competition outcome.
Scanning electron micrographs and light microscopy photographs (Figs. 2 and 3, respectively) show the morphological differences in the mutant strains when compared to the wild-type strain and to one another.
The absence of obvious correlation between levels in lateral and central domains highlights qualitative differences in the mutants.
Thus, evaluating this set of mutants provides a potentially sensitive analysis for subtle effects of loss of exocyst function, i.e. small differences in the mutants can be considered more credible when the magnitude of those differences consistently correlate with the severity of the root growth defect across all mutants evaluated.
No difference was seen in the mutant glands, compared to the non feeding WT glands at L1 or L3 (data not shown).
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Justyna Jupowicz-Kozak
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