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In addition, a clear difference in the internalization amount can be observed from cell samples fed with prickly and round NDs —the amount of the latter NDs was ~4 times higher than that of the former NDs.
Such a phenomenal difference in the internalization of Aβ40 between neurons and BBB endothelial cells may provide essential clues to understanding how various cells can differentially regulate Aβ proteins and help explain the vulnerability of cortical and hippocampal neurons to Aβ toxicity.
We found that there was no significant difference in the internalization of solid discs by J774A.1 macrophages compared to solid spheres after 2 h of incubation.
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The data reveal striking differences in the internalization behaviour and gene silencing efficiency in the tested cell lines, despite their common lung epithelial origins.
In addition, marked differences in the internalization efficiency of the 18F-labeled EuE-based inhibitors in comparison to the two reference compounds 18F-DCFPyl and 18F-PSMA-1007 were observed (Fig. 2a).
Differences in the internalization of F-Aβ40 and AF633-Trf, controlled for temperature and energy dependent transport, was also assessed using laser confocal microscopy and flow cytometry.
The differences in the internalization kinetics may suggest that ES and its hybrid variants use different endocytic routes.
Contrary to CysLT2, LTD4 did not induce internalization of hGPR17-L as only constitutive internalization was observed when either this or vehicle was present with no apparent differences in the internalization pattern between these conditions.
Although cross-linking of FPR molecules at the cell surface by the antibodies is likely to enhance receptor internalization, this approach could still allow the detection of clear differences in the internalization between stimulated and non-stimulated cells.
It also remains possible that different antibody isotypes 44 give rise to the difference in AMPAR internalization, because the patient antibodies are mainly but not exclusively IgG, commercial anti-GluA1 is polyclonal IgG, and commercial anti-GluA2 is monoclonal IgG2a.
A possible explanation for the kinetic difference in receptor internalization is that the WT and mutant receptors use a different pathway for internalization.
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