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There was no difference in the extraction of tenascin X from wild type and Matn3 V194D cartilages, which was consistent with LC-MS/MS data.
A further difference in the extraction of a collagen-interacting protein was observed in Comp DelD469 cartilage extractions, where there was a significant decrease in the detection of tenascin X compared with other genotypes.
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This could be due to the differences in the extraction methods and nature of extracts employed.
Significant differences in the extraction efficiency were caused by the different extraction procedures and disintegration strategies (Fig. 2).
Animal and regional differences in the extraction efficiency of doxorubicin from the different tumors may also affect the ability to precisely quantify permeability.
SDS-PAGE and Western blot analysis of sequentially extracted knee cartilage revealed genotype-specific differences in the extraction of a number of proteins that included matrilin-1 to -4, COMP and type IX collagen, which are all known to interact with each other (Budde et al., 2005; Fresquet et al., 2010; Fresquet et al., 2007; Holden et al., 2001; Mann et al., 2004; Wiberg et al., 2003).
In order to identify both commonalities and differences in the extraction profiles of structural ECM components from the three different mouse models of PSACH-MED, we compared the different MS-derived peptide profiles (unweighted spectra number) (supplementary material Tables S1 S3) from mice of all three genotypes (Fig. 4).
Increasing irrigation rates produced no differences in total biomass and also no differences in the extraction of heavy metals.
No significant differences in the extraction efficiency of the fat content in bakery samples using FMASE versus the official method were found.
Response surface methodology revealed optimal PLE parameters for polyphenols recovery, observing differences in the extraction conditions (number of cycles, temperature and time) according to the specific polyphenol groups.
Differences in the extraction curves were interpreted as a weaker affinity for fluoride ion and the formation of the hexafluororutherfordate ion, whereas hafnium and zirconium ions complex seven or eight fluoride ions at the concentrations used: : + 6 →.
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