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A number of comparative studies demonstrated a difference in the differentiation capacity of MSCs from specific tissues.
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To facilitate comparisons between experiments, we used the fractional enhancement (Fr enhance) metric, where the compound-driven difference in Th differentiation is normalized against the difference between the positive and negative controls within the experiment (i.e., Thhi − Thlow).
In contrast, the hDiffModule cannot distinguish between differentiated cells of different types but is able to predict differences in the differentiation potential of pluripotent cells of different origins.
However, several differences in the differentiation potential were noted using the two different protocols.
The discrepancy with respect to mir-503 regulation may relate to differential regulation between man and mouse, or differences in the differentiation protocols applied.
We did not see differences in the differentiation and clonogenic potential of trehalose-loaded HPC after freezing and thawing compared to control groups.
This difference in culture conditions and, therefore, in possible differences in the differentiation state of recorded cells may help to explain why our results and those of Liebau et al. [4] differ.
The use of peptide pools as antigenic stimulation did not allow us to detect differences in the differentiation status of HIV-1-specific CD8+ T cells between Controllers and Progressors (p = 0.44 Fisher's exact, data not shown).
The results revealed differences in the differentiation patterns and the capacity between cells obtained from examined tumors.
Conversely, Lac/Cr did not show statistically significant differences in the differentiation between adenomas and nonadenomas (P = 0.063).
Therefore, in this study, we precisely analyzed the differences in the differentiation potentials between CD34+/− HSPCs in vivo and in vitro.
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