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There is a small difference in the curves of simulated cases with respect to the experimental ones.
Examination of the SALI curves (Figure 5a) reveals a qualitative difference in the curves models tested 2D vs. 3D descriptors.
Although MCP-1 markedly increased amylin mRNA level, there was no significant difference in the curves of mRNA decay between MCP-1 treated and control groups (Figure 1E), suggesting that MCP-1 increase amylin expression at transcriptional level.
Post-hoc comparisons using paired t-tests showed that the relative difference in the curves became significant starting with the 4th pulse (Bonferroni-adjusted level of significance of P<0.0033).
Examining the data closely, we can see that there are three aspects that characterise the difference in the curves.
ROC curve analysis of data utilising only single samples from each infant produced no significant difference in the curves (data not shown).
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As such tests yielded no major difference in the curve shapes, we kept a full model.
Kaplan-Meier product limit method was used to estimate cumulative probability curve for time to first exacerbation in each treatment group and log rank test was used to detect group difference in the curve.
This was done to assure that differences in the curves would be due to different severity stage assignments only, and not to other differences (e.g. demographic differences) between the groups.
Therefore, differences in the curves are due to iron.
The differences in the curves for substrate and cofactor is similar to other tyrosine hydroxylases and is likely to indicative of the biochemical mechanism of catalysis as discussed below (25).
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