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We therefore conclude that the reported difference in the assay performance between the two SmD peptide assays is mainly attributed to the different definitions of the cut-off.
A key difference in the assay conditions used for the native and recombinant AMPK (e.g., Figures 2A and 2C) was that, while the former was performed with the kinase in solution, the latter was performed with the recombinant myc-tagged kinase coupled to anti- myc antibodies, which was necessary to remove it from the endogenous AMPK in the cells used for expression.
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There was little difference in the assays' ability to detect prostate mRNA in advanced PC patients.
Another important difference in the assays is the fact that the bulk saccharification assays involved shaking/mixing whereas the NR study was performed in the absence of mixing.
However, a study on diagnostic methods for brucellosis where IgG and IgM antibodies were measured simultaneously found little difference in the assays.
Although many factors are likely to be contributory, differences in the assay design are of key importance.
Thus, the discrepancy may due to differences in the assay conditions.
This can be explained by the profound differences in the assay formats.
The lower anti-PEG titers appeared to relate to differences in the assay such that interference by pegloticase in the test sera markedly reduced Ab titers.
The gap between the classical MPF and the current MPF thus reflects differences in the assay systems employed and quantification of cyclin B-Cdk1 activity.
n.t. is not tested as this laboratory was not participating in the indicated phase In order to identify critical parameters responsible for variability, we then focused on the differences in the assay protocols used by the participating laboratories.
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