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Despite the striking difference in the absolute amounts of morphine consumed, the behavioural activation induced by morphine self-administration was similar in WT and RMOR mice across time (Fig 2D, time effect only: F[4,88] = 7.6, p < 0.0001).
There was no statistical difference in the absolute amounts of urine GM-CSF, IFN-γ, IL-2, IL-4, IL-12, and IL-18 among groups (data not shown).
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At the same time, this normalization preserves strain-to-strain differences in the absolute amounts of individual or summed FA components, since dry mass was accurately measured for lyophilized samples, and loads were thus standardized prior to GC-MS analysis.
In contrast, significant differences in the absolute amounts of CER were found among 6 of the 8 body sites tested (P < 0.01, Friedman test) with higher amounts at the metacarpus, axillary region and lateral thorax and lower amounts at the pinna, lip and caudal back (see Additional file 1).
The vitamin C in the multiple micronutrient supplements might have enhanced the absorption of iron and also contributed to reducing the difference in the absolute amount of iron absorbed.
As expected, overall uptake was low and there was a marked difference in the absolute amount of NP uptake in the presence and absence of serum (∼2-fold higher uptake without serum).
In effect, the analysis of modulated data tests for regional differences in the absolute amount (volume) of grey matter.
In addition, we performed a volume change correction (modulation) by modulating each voxel with the Jacobian determinants derived from spatial normalization, which allowed us to determine regional differences in the absolute amount of brain tissue (Ashburner and Friston 2000).
ChIP qPCR confirmed that there were indeed no major differences in the absolute amount of wild-type and mutant cohesin complexes associated with a variety of loci (Supplementary Figure 7C).
No difference was observed in the absolute amount of T cells in the circulation [1.37×106 (0.91–2.39) vs. 1.32×106 (0.90–2.24) cells/mL; P = 0.761). PBMC were stained directly ex vivo to determine the proportions of circulating CD4+ Treg cells (Figure 2A). Week 0 and week 12 proportions of CD25+FoxP3+ and CD25+CD127− CD4+ Treg did not differ significantly (Figure 2B C).
The only significant baseline difference between the groups was in the absolute amount of visceral fat (Fig. 1 B), which was greater in those who did not lose the diagnosis (166 ± 49 vs. 132 ± 33 cm, P = 0.04).
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