The difference in staining pattern may reflect different PODXL function in cancer compared to normal tissue.
There was no significant difference in staining patterns between the two different anti-CCR2 antibodies.
To determine the difference in staining intensity for total and phospho Akt, 10 different but histologically similar fields were selected per sample and the slides were analyzed using NIH image J software.
No notable difference in staining intensity or in the cross-reactivity of different cell types was evident for the stages examined (days 19 26 of the 26.5 day gestation).
On visual inspection, no difference in staining intensity at the surface of the implant was noted, comparing different experiments or when the hydrogel was used alone or mixed with 2% vancomycin.
However, Donors D and E did not exhibit an appreciable difference in staining with depolarization (Fig. 2D,E), which is also consistent with the gene expression data that indicated a lower degree of response in these cell lines.
Evaluation of lipid accumulation by Oil Red O staining also revealed that Donor E cells did not exhibit an appreciable difference in staining between cells differentiated with or without HK.
The BAX protein was found to be strongly expressed in all tumors, with no difference in staining intensities between experimental groups.
In 2014 and 2015, Rimm and his team used QIF to assess whether they could detect a difference in staining with antibodies targeting the HER2 intra- or extracellular domains.
In contrast, there was no difference in staining for podocin, used as a control.
We observed no difference in staining between photolabelled and non-labelled nuclei.
