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Studies in several species of bacteria have established that there is a log-linear decrease in recombination frequency with increasing difference in sequence between host and recipient DNA.
Notably, disruption of ERE2 alone did not completely abolish the responsiveness to doxorubicin even without ER ectopic expression, possibly related to the difference in sequence between ERE1 and ERE2 and their relative closeness to the ER consensus.
The only difference in sequence between the two transcripts is that the JM-a isoform is characterized by the insertion of a 75-nt sequence derived from exon 16, whereas JM-b is encoded by the alternative 45-nt sequence.
The difference in sequence between members of a family of transposons during the bursts is between approximately 1percent and 144 percent, depending on the family.
Sequencing PCR products (obtained with primers "B" in Fig. 2a) at the exon 5-exon 6-junction revealedevealed no difference in sequence between the wild type and the mutant (data not shown).
There was little difference in sequence between the two resistant phenotypes, with only 330 differential SNPs (including 14 non-synonymous changes) affecting 130 genes, none of them being candidate genes (Additional file 6: Table S6).
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A similar analysis with I-1 reveals multiple differences in sequence between it and all other seven haplotypes.
Their unsuitability is probably explained by differences in sequence between the primers and the new variant types [ 45, 46].
Overall, there are 26 differences in sequence between BgD and HgD, some of which may be more important than others.
We used Sanger sequencing to validate our NGS data, including sequencing of the inverted repeat junctions, regions of ambiguous sequence, SNPs, and differences in sequence between Illumina and 454 approaches.
However, based on the differences in sequence between the luminal parts of yeast IRE1 and mammalian IRE1 α[ 10], the differences in their structure [ 16, 17], and their differential abilities to prevent unfolded protein aggregation [ 18, 19], we model the activation of the mammalian IRE1 α as dependent only on BiP.
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