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It is possible that the difference between our flow cytometry data and previous report data [ 10] was because of the difference in passage number.
In the present study, no differences in muscle and liver gene transcription among fish groups were found, which suggests that energy was not the main cue explaining the difference in passage behavior.
Although given the small difference in passage rates (6 12 hours) and limited movements of polar bears on land [ 33, 45], we feel it is more likely due to the widespread occurrence and/or mobile nature of the land-based animals that polar bears consume.
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The fact that two sensitive sites did not show the expected drop in peak heights is probably due to different growth conditions, differences in passage numbers or to our batch of HeLa cells being different from the one used in the ENCODE study rather than to limitations of our method.
A point of note, in my experience differences in passage number of the cells used for differentiation can affect gene expression levels throughout.
No substantial species differences in passage number or estimated population doubling level distinguish the cells used in this study (fig. 4 C), suggesting that these factors are unlikely to account for the phenotypic difference between species.
Other potential confounders include: unmatched age, gender and ethnicity between the patients and control individuals; differences in methodology used to induce pluripotency (e.g. lentivirus versus RNA transfection); and differences in passage number and adaptation to culture of the iPSC lines.
Eventually, it can be seen that dipole patterns appear between the southeastern region and the northeastern region of East Asia from the difference in TC passage frequencies between the two groups.
There was no difference in serial passaging of MSC-CM derived spheres and untreated MCF-7 mammospheres (data not shown).
In M. pulmonis infections, the TLR2−/− mice had 2-log higher M. pulmonis numbers in the lungs when compared to their WT counterparts, but there was no difference in nasal passages.
In addition, differences in TC passage frequencies between the two groups were analyzed (Fig. 4b).
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