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This can be explained by the difference in masses of Cd and Zn ions: different energies are needed for the excitement of the light Zn and heavy Cd ions.
The difference in masses between these two peaks, equal to unmodified α-synuclein plus the equivalent mass of three nitrate groups, indicates that all three tyrosine residue acceptor sites in the SYN protein possibly had been nitrated.
A valid question is therefore whether the observed difference in masses for these particular peaks is real or an artefact related to instrument drift over time.
The first one was the primary alcohol since the difference in masses between IL2 ion and this ion was 15.9979 (mass of an oxygen atom) and indicated the transformation of the –CH3 moiety into –CH2OH (CH2OH9ON2).
The difference in masses between IL2 ion and this ion is 13.9817, which indicates the addition of one oxygen atom and the removal of two hydrogen atoms: –CH3 vs. –CHO (C10H17ON2).
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Either there are two different N-glycan species with a difference in mass of roughly 1 Da, or there are two forms of the same N-glycan.
We also observed a significant difference in mass between ad libitum animals on different formulation of diet, LRD and PD.
The difference in mass becomes chemically important in certain instances.
However, subtle chemical effects do result from the difference in mass of isotopes.
The difference in mass is often expressed as energy by using Albert Einstein's relativity equation in the form E = (Δm c2.
Moreover, all the isotopes of a given chemical element are nearly identical except for a very small difference in mass.
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