We were able to exclude differences in cultured medium as an explanation for the lower fold-inductions in human hepatocytes (data not shown).
Despite these differences in cultured cells, MDk/W452(H5N8) replicated in human nasal respiratory epithelium and lung tissues as well as the HPAI A(H5N1) viruses did ex vivo (3.0 4.0 log10EID50/g maximum titers).
Identifying gene expression differences in cultured MPNST cell lines, NFSCs and normal Schwann cells avoided the complex heterogeneity inherent to solid tumours; using the cell culture gene signature to identify common gene expression patterns, in the solid tumours, which allowed us to identify the Schwann cell component.
Some studies reported no significant difference in the cultured bacteria between patients with and without prior antibiotic treatment [ 14, 23, 28].
In addition, expression levels of IL8Rβ are shown as an example of an invariant control (no difference in between cultured non-malignant endothelial cells and hemangiosarcoma cells), and GAPDH and β-actin were used as housekeeping controls to confirm that the normalization strategy was valid.
The PCA results show that the total proteome signal from the different stages, when considered as individual matrices, cluster far apart from each other, confirming that the berry cultures originating from berry explants in different developmental stages, maintained significant differences in the cultured state.
In agreement with previous works, EDCs are a distinct population of small, round highly refractile cells which appeared 1 3 weeks after cardiac explants were cultured despite some differences in culture conditions.
In contrast, the native and invasive strain showed no significant difference in replication in cultured mosquito, monkey or human cells.
The purpose of the present study was to clarify the expression levels of transporter proteins in human lung tissue and to analyze regional and interindividual differences in primary cultured epithelial cells.
However, many physiological processes are three-dimensional in nature and recent studies have shown morphological differences in cells cultured on two-dimensional substrates versus three-dimensional matrices.
