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Conclusions: Acknowledging the difference in assay methods used for HCMV DNA quantitation, the in-house PCR PBL assay and the COBAS Amplicor HCMV monitor plasma assay predicted equally well for HCMV pneumonitis in LTR.
However, they make a large difference in assay performance.
There was no difference in assay performance detectable between these groups.
The difference in assay performance by week of illness onset was not significant (p>0.05, by χ test).
This is because at higher biomarker frequencies, the difference in assay cost makes minimal difference to the overall cost-effectiveness of the screening-treatment pairing.
Difference in assay conditions, such as the presence of detergents, can affect the molecular interactions necessary for PS1 complex formation and its subsequent detection [ 10].
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Since we used PPTs during the entire RT-PCR and bDNA periods, collection tube effects likely influenced RT-PCR and bDNA assays differently, contributing to the observed differences in assay reliability near the LLOQ.
Alternatively, differences in HEV prevalence detected in livers and that detected in feces may be caused by differences in assay sensitivity or different distribution patterns in the 2 sample matrices.
This might reflect different hormone concentrations in urine versus blood, differences in assay sensitivity, or other methodological differences concerning sample collection.
These include different specificities, efficiencies and mechanisms of action of the treatments as well as differences in assay platforms.
Adjustment of findings for differences in assay sensitivity indicated that these estimates may be conservative.
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