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The absolute value of the difference between the replicated sample size calculation and the authors' target sample size was greater than 10% for 47 articles (30%) and greater than 50% for 10 (6%).
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For this trait, there was no loss of data and no significant differences between the replicated plots.
No qualitative difference between the replicates of each extract was found so the four spectra (two replicate FT-IR measurements of two extractions per solvent) were averaged.
The ANOVA F-values of SHAP tasks and the FP and IOF functionality scores do not exceed Fcritical = 3.28, indicating there is no difference between the replicates and demonstrating, thus, good reliability [ 1, 28].
The standard deviation of the difference between these replicate recordings in the aorta was ±5.9 mmHg for mean blood pressure (5% within subject coefficient of variation) and ±0.31 ms−1 for mean Doppler velocity (16% within subject coefficient of variation).
Systematic variation of only two factors was sufficient to mimic the range of differences between the replicate experiments, resulting in almost perfect reproducibility [34].
For each variable, the cluster effect was expressed as the mean of the relative differences between the replicates of the strains within a given cluster and the control without bacteria.
(5) ϵ t = 〈 | x t − μ t | 〉 = ∫ | x t − μ t | p (x ) d N x (6) p (x | { μ t, ϵ t } ) = ∏ t = 1 m 1 2 ϵ t exp (− | x ˜ t − μ t | ϵ t ) Equation (5) defines the normalizing constant ϵ t as the expected value of the moduli of the differences between the replicate observations at time t and the values predicted by the kinetic model (μ t).
For performing one-way ANOVA test, normality and equal variance must be considered; the difference between the five replicates must be insignificant and the model designed by the software should be reliable.
In case of excessive difference between the two replicates (one Ct or more), the run was repeated.
The numerically small but statistically significant difference between the two replicates (see raw data for individual variables) may be attributed to the differences in test setups and conditions.
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