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It can be seen in Table 2 the smallest difference between the cycle slip candidate pairs is (±9, ±7), namely 9 cycles on L1 frequency and 7 cycles on L2 frequency.
Delta CT values were defined as the absolute difference between the cycle threshold (CT) of HMGB1 RNA and the house-keeping gene encoding 18S rRNA.
That is, for each gene and sample, a normalized expression was calculated as the difference between the cycle threshold (CT) value of the gene and the corresponding CT of the endogenous control.
ΔCt stands for the difference between the cycle threshold of the target gene and that of the endogenous control genes.
RNA expression was quantified by calculating the difference between the cycle threshold of the mRNA of interest, and the reference gene (18s mRNA) for each sample.
Using the delta cycle threshold (ΔCt) method, which measures the difference between the cycle time of the biomarker and that of a reference gene, a normalised ΔCt value for EREG expression relative to GAPDH expression was determined for each sample.
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Abundance of mRNA in each sample was determined by the differences between the cycle threshold (Cτ) values for each genes and β-actin, ΔCτ.
No statistically significant differences between the cycle efficiency averages of the competitor and target sequences were encountered using a paired t test (p < 0.05).
Differences between the cycle threshold (Ct) of the target gene and the Actin gene were used to obtain relative transcript levels of the target gene, and calculated as 2 exp- Cttarget - Ctactin).
The principal difference between the Clerk cycle and the more common Otto cycle is that the Clerk cycle generates an ignition once every two strokes of the piston rather than once every four.
ΔCt = Ct BCG) - Ct GAPDH), which means the difference between the threshold cycle of BCG and the threshold cycle of the corresponding GAPDH in the same sample.
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