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It is also important to confirm that the FSC/SSC profiles of the 2 sorted populations are not different; this could indicate that the sorted cells actually differ in their size making it look like there are differences in flux that do not actually exist.
For instance, titin co-expresses in the heart as two main isoforms, N2BA and N2B, that differ in their size and mechanical properties1.
Both ZA-LDH and ZA had a prominent layered shape; however, they differ in their size by about 40 nm.
These results show that wt and m8 proteins form two aggregates that differ in their size (the wt protein forming on average larger aggregates than m8) and in their biochemical properties (resistance to detergent).
As the sequence sets considerably differ in their size, we make these distributions comparable by computing ratios.
The EPMs in Eγ differ in their size and shape as well as in their structural positions in both RNAs.
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Four varieties of P. pusio are known, which differ in their sizes, spore morphology, and distribution.
These findings indicate that although the deposits differ in their sizes, internal distribution and microstructure, their chemical composition is the same.
While both ParAc and ParAm are Walker-type ATPases which contain a conserved P-loop ATP binding motif, they differ in their sizes (249 aa for ParAc and 322 aa for ParAm) as well as in the presence of an N-terminal helix-turn-helix motif (HTH) in ParAm, a feature that normally appears in plasmid ParAs but not in their chromosomal counterparts [ 21].
Both observations are explained by partial cocrystallization, i.e. formation of crystallites consisting of two populations of crystallites differing in their size, perfection, origin and time of appearance.
This study underlined also the potentiality of using molecules differing in their size as an original way to probe the architecture of these systems.
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